Adsorption of the inulinase from Kluyveromyces marxianus NRRL Y-7571 on Streamline® DEAE resin
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منابع مشابه
ADSORPTION OF THE INULINASE FROM Kluyveromyces marxianus NRRL Y-7571 ON STREAMLINE DEAE RESIN
The enzyme inulinase is used to produce oligosaccharides and fructose, with up to 95% fructose in a single stage of inulina hydrolysis. With in the aim to purify the enzyme, studies on the conditions of enzyme adsorption in an expanded-bed column were conducted using phosphate and tris-HCl buffers. The inulinase used in this work was obtained from Kluyveromyces marxianus NRRL Y-7571 by fermenta...
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In this work, the recovery and purification of inulinase produced by Kluyveromyces marxianus NRRL Y-7571 in solid state fermentation using in aqueous two-phase systems (ATPS) was studied. The effects of concentration of PEG and potassium phosphate were evaluated using experimental design technique. The SAB extraction yielded a purification factor (PF) up to 3.85-fold, using 18% PEG 4000 and 7% ...
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Various carbon sources were evaluated for production of inulinase by yeast, Kluyveromyces marxianus MTCC 3995. Highest inulinase activity was observed with Dahlia extract (25.3 nkat mL(-1)) as carbon source. The enzyme activity was 1.4 folds higher than that observed in media containing pure chicory inulin (17.8 nkat mL(-1)). The yeast showed good growth on a simple medium containing dahlia ext...
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Kluyveromyces marxianus var. marxianus was found to secrete a large amount of extracellular inulinase in to the medium. The optimization of inulinase production using copra waste as a carbon source was performed with statistical methodology based on experimental designs. The screening of eighteen nutrients for their influence on inulinase production was achieved using a Plackett– –Burman design...
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The present study was conducted to investigate the influence of initial sucrose concentration, pH and aeration rate on biomass and inulinase production by Kluyveromyces marxianus var. bulgaricus in a stirred batch reactor. Maximum inulinase activity (15.29 UmL) was obtained at a sucrose concentration of 10 g L, pH 5.0 and aeration rate of 1 vvm. The 20 g L sucrose concentration was suitable for...
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ژورنال
عنوان ژورنال: Brazilian Journal of Chemical Engineering
سال: 2005
ISSN: 0104-6632
DOI: 10.1590/s0104-66322005000400006